Publications
2022
Brameyer S, Schumacher K, Kuppermann S, Jung K (2022) Division of labor and collective functionality in Escherichia coli under acid stress. Commun Biol 5: 327.
Burkart T*, Wigbers MC*, Würthner L*, Frey E (2022) Control of protein-based pattern formation via guiding cues, Nat Rev Phys 4, 511–527.
Czech L, Mais CN, Kratzat H, Sarmah P, Giammarinaro P, Freibert SA, Esser HF, Musial J, Berninghausen O, Steinchen W, Beckmann R, Koch HG, Bange G (2022) Inhibition of SRP-dependent protein secretion by the bacterial alarmone (p)ppGpp. Nat Commun 13: 1069.
Kratzat H, Mackens-Kiani T, Ameismeier M, Potocnjak M, Cheng J, Dacheux E, Namane A, Berninghausen O, Herzog F, Fromont-Racine M, Becker T, Beckmann R (2022) A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes. EMBO J 40: e105179.
Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR (2022) Ribosome collisions induce mRNA cleavage and ribosome rescue in bacteria. Nature 603: 503-508.
Sattler L, Graumann PL (2022) Assembly of Bacillus subtilis dynamin into membrane-protective structures in response to environmental stress is mediated by moderate changes in dynamics at a single molecule level. Microb Physiol 10: 1-13.
Schwarz J, Schumacher K, Brameyer S, Jung K (2022) Battle of bacteria against acidity, FEMS Microbiol Rev (in press, doi: 10.1093/femsre/fuac037)
2021
Babl L, Giacomelli G, Ramm B, Gelmroth A, Bramkamp M, Schwille P (2021) CTP-controled liquid-liquid phase separation of ParB. J Mol Biol 434: 167401.
Feddersen H, Würthner L, Frey E, Bramkamp M (2021) Dynamics of the Bacillus subtilis Min system. Mbio 12: 2:e00296-21.
Hanauer C, Bergeler S, Frey E, Broedersz CP (2021) Theory of active intracellular transport by DNA relaying. Phys Rev Lett 127: 138101.
Heermann T*, Steiert F*, Ramm B, Hundt N, Schwille P (2021) Mass-sensitive particle tracking to elucidate the membrane-associated MinDE reaction cycle. Nat Methods: 1239-1246.
Krol E, Stuckenschneider L, Kästle Silva JM, Graumann PL, Becker A (2021) Stable inheritance of Sinorhizobium meliloti cell growth polarity requires an FtsN-like protein and an amidase. Nat Commun 12: 545.
Martini L, Brameyer S, Hoyer E, Jung K, Gerland U (2021) Dynamics of chromosomal target search by a membrane-integrated one-component receptor. PLoS Comput Biol 17: e1008680.
Mayer B, Schwan M, Oviedo-Bocanegra LM, Bange G, Thormann KM, Graumann PL (2021) Dynamics of bacterial signal recognition particle at a single molecule level. Front Microbiol 12: 663747.
Messelink JJ, Meyer F, Bramkamp M, Broedersz CP (2021) Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth. Elife 10: e70106.
Messelink JJ, van Teeseling MC, Janssen J, Thanbichler M, Broedersz CP (2021) Learning the distribution of single-cell chromosome conformations in bacteria reveals emergent order across genomic scales. Nat Commun 12: 1-9.
Mayer B, Schwan M, Thormann KM, Graumann PL (2021) Antibiotic drug screening and image characterization toolbox (A.D.I.C.T.): a robust imaging workflow to monitor antibiotic stress response in bacterial cells. F1000 Res 10: 277.
Oviedo-Bocanegra L, Hinrichs R, Rotter D, Dersch S, Graumann PL (2021) Single molecule/particle tracking analysis program SMTracker 2.0 reveals different dynamics of proteins within the RNA degradosome complex in Bacillus subtilis. Nucleic Acids Res 49: e112.
Osorio-Valeriano M, Altegoer F, Das CK, Steinchen W, Panis G, Connolley L, Giacomelli G, Feddersen H, Corrales-Guerrero L, Giammarinaro PI, Hanßmann J, Bramkamp M, Viollier PH, Murray S, Schäfer LV, Bange G, Thanbichler M (2021) The CTPase activity of ParB determines the size and dynamics of prokaryotic DNA partition complexes. Mol Cell 81: 3992-4007.
Ramm B*, Goychuk A*, Khmelinskaia A, Blumhardt P, Eto H, Ganzinger K, Frey E, Schwille P (2021) A diffusiophoretic mechanism for ATP-driven transport without motor proteins. Nat Phys 17: 850-858,
Sattler L, Graumann PL (2021) Real time messenger RNA dynamics in Bacillus subtilis. Front Microbiol 12: 760857.
Schumacher D, Harms E, Bergeler S, Frey E, Søgaard-Andersen L (2021) PomX, a ParA/MinD ATPase activating protein, is a triple regulator of cell division in Myxococcus xanthus, Elife 10, e66160.
Tostevin F, Wigbers M, Søgaard-Andersen L, Gerland U (2021) Four different mechanisms for switching cell polarity. PLoS Comput Biol 17: e1008587.
Williams MA, Aliashkevich A, Krol E, Kuru E, Bouchier J, Rittichier J, Brun Y, VanNieuwenhze M, Becker A, Cava F, Brown PJB (2021) Unipolar peptidoglycan synthesis in the Rhizobiales requires an essential class A penicillin-binding protein. Mbio 12: e0234621.
2020
Anand D, Schumacher D, Søgaard-Andersen L (2020) SMC and the bactofilin/PadC scaffold have distinct yet redundant functions in chromosome segregation and organization in Myxococcus xanthus. Mol Microbiol 114: 839-856.
Biselli E, Schink SJ, Gerland U (2020) Slower growth of E. coli leads to longer survival in carbon starvation due to a decrease of the maintenance rate. Mol Syst Biol 16: e9478.
Blagotinsek V, Schwan M, Steinchen W, Mrusek D, Hook J, Rossmann F, Freibert SA, Kratzat H, Murat J, Kressler D, Beckmann R, Beeby M, Thormann K, Bange G (2020) An ATP-dependent partner switch links flagellar C-ring assembly with gene expression. Proc Natl Acad Sci USA 117: 20826-20835.
Böhm K, Giacomelli G, Meyer F, Bramkamp M (2020) Chromosome organization and cell growth of Corynebacterium glutamicum. In: Corynebacterium glutamicum: Biology and Biotechnology, edited by Inui M & Toyoda K, 2nd ed., Springer, New York, pp. 3-24.
Böhm K, Giacomelli G, Schmidt A, Imhof A, Koszul R, Marbouty M, Bramkamp M (2020) Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum. Nat Commun 11: 1485.
Brameyer S, Hoyer E, Bibinger S, Burdack K, Lassak J, Jung K (2020) Molecular design of a signaling system influences noise in protein abundance under acid stress in different γ-Proteobacteria. J Bacteriol 220: e00121-20.
Brauns F*, Halatek J*, Frey E (2020) Phase-space geometry of mass-conserving reaction-diffusion dynamics, Phys Rev X 10: 041036.
Corrales-Guerrero L, He B, Refes Y, Panis G, Bange G, Viollier PH, Steinchen W*, Thanbichler M* (2020) Molecular architecture of the DNA-binding sites of the P-loop ATPases MipZ and ParA from Caulobacter crescentus. Nucleic Acids Res 48: 4769-4779. *co-corresponding authors
Brauns F, Halatek J, Frey E (2020) Phase-space geometry of mass-conserving reaction-diffusion dynamics. Phys Rev X 10: 041036.
Carreira LAM, Tostevin F, Gerland U, Søgaard-Andersen L (2020) Protein-protein interaction network controlling establishment and maintenance of switchable cell polarity. PLoS Genet 16: e1008877.
David G, Walter JC, Broedersz CP, Dorignac J, Geniet F, Parmeggiani A, Walliser NO, Palmeri J (2020) Phase separation of polymer-bound particles induced by loop-mediated one dimensional effective long-range interactions. Phys Rev Res 2: 033377.
Dersch S, Mehl J, Stuckenschneider L, Mayer B, Roth J, Rohrbach A, Graumann PL (2020) Super-resolution microscopy and single-molecule tracking reveal distinct adaptive dynamics of MreB and of cell wall-synthesis enzymes. Front Microbiol 11: 1946.
El Najjar N, Geisel D, Schmidt F, Dersch S, Mayer B, Hartmann R, Eckhardt B, Lenz P, Graumann PL (2020) Chromosome segregation in B. subtilis follows an overall pattern of linear movement and is highly robust against cell cycle perturbations. mSphere 5: e00255-20.
El Najjar N, van Teeseling MCF, Mayer B, Herrmann S, Thanbichler M, Graumann PL (2020) Bacterial cell growth is arrested by violet and blue, but not yellow light excitation during fluorescence microscopy. BMC Mol Cell Biol 21: 35.
Fröhlich KS, Papenfort K (2020). Regulation outside the box: New mechanisms for small RNAs. Mol Microbiol 114: 363–366.
Giunta G, Seyed-Allaei H, Gerland U (2020) Cross-diffusion induced patterns for a single-step enzymatic reaction. Commun Phys 3: 167.
Heermann T, Ramm B, Glaser S, Schwille P (2020) Local self enhancement of MinD membrane binding in Min protein pattern formation. J Mol Biol 432: 3191-3204.
Herzog R, Peschek N, Sprenger M, Singh PK, Fröhlich KS, Schröger L, Meyer F, Bramkamp M, Drescher K, Papenfort K (2020) RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae. Nat Commun 11: 1-11.
Hook JC, Blagotinsek V, Pané-Farré J, Mrusek D, Altegoer F, Schier L, Thormann KM, Bange G (2020) A proline-rich element in the type III protein FlhB contributes to flagellar biogenesis in the beta- and gamma-proteobacteria. Front Microbiol 11: 2908.
Krol E, Yau HCL, Lechner M, Schäper S, Bange G, Vollmer W, Becker A (2020) Tol-Pal system and Rgs proteins interact to promote unpolar growth and cell division in Sinorhizobium meliloti. mBio 11: e00306-20.
Mazzantini D, Fonnesu R, Celandroni F, Calvigioni M, Vecchione A, Mrusek D, Bange G*, Ghelardi E* (2020) GTP-dependent FlhF homodimer supports secretion of a hemolysin in Bacillus cereus. Front Microbiol 11: 879.
*co-corresponding authors
Miermans CA, Broedersz CP (2020) A lattice kinetic Monte-Carlo method for simulating chromosomal dynamics and other (non-)equilibrium bio-assemblies. Soft Matter 16: 544-556.
Müller-Esparza H, Osorio-Valeriano M, Steube N, Thanbichler M, Randau L (2020) Biolayer interferometry analysis of the target binding activity of CRISPR-Cas effector complexes. Front Mol Biosci 7: 98.
Nürenberg-Goloub E, Kratzat H, Heinemann H, Heuer A, Kötter P, Berninghausen O, Becker T, Tampe R, Beckmann R (2020) Molecular analysis of the ribosome recycling factor ABCE1 bound to the 30S post-splitting complex. EMBO J 39: e103788.
Peschek N, Herzog R, Singh PK, Sprenger M, Meyer F, Frohlich KS, Schroger L, Bramkamp M, Drescher K, Papenfort K (2020) RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae. Nat Commun 11: 6067
Petersen I, Schlüter R, Hoff KJ, Liebscher V, Bange G, Riedel K, Pané-Farré J (2020) Non-invasive and label-free 3D-visualization shows in vivo oligomerization of the staphylococcal alkaline shock protein 23 (Asp23). Sci Rep 10: 125.
Pinheiro B, Petrov DP, Guo L, Benevides Martins G, Bramkamp M, Jung K (2020) Elongation factor P is required for EIIGlc translation in Corynebacterium glutamicum due to an essential polyproline motif. Mol Microbiol 115: 320-331.
Potapova A, Menezes Carreira LA, Søgaard-Andersen L (2020) The small GTPase MglA together with the TPR domain protein SgmX stimulates type IV pili formation in M. xanthus. Proc Natl Acad Sci USA 117: 23859-23868.
Romero H, Serrano E, Hernández-Tamayo R, Carrasco B, Cárdenas PP, Ayora S, Graumann PL, Alonso JC (2020) Bacillus subtilis RarA acts as a positive RecA accessory protein. Front Microbiol 11: 92.
Schibany S, Hinrichs R, Hernández-Tamayo R, Graumann PL (2020) The major chromosome condensation factors Smc, HBsu, and Gyrase in Bacillus subtilis operate via strikingly different patterns of motion. mSphere 5: e00817-20.
Weng TH, Steinchen W, Beatrix B, Berninghausen O, Becker T, Bange G, Cheng J, Beckmann R (2020) Architecture of the active post-translational Sec translocon. EMBO J 40: e105643.
2019
Carillo M, Wagner M, Petit F, Dransfeld A, Becker A, Erb T (2019) Design and control of extrachromosomal elements in Methylorubrum extorquens AM1. ACS Synth Biol 8: 2451–2456.
Najafi J, Altegoer F, Bange G, Wagner C (2019) Swimming of bacterium Bacillus subtilis with multiple bundles of flagella. Soft Matter 15: 10029-10034.
Osorio-Valeriano M, Altegoer F, Steinchen W, Urban S, Liu Y, Bange G*, Thanbichler M* (2019) ParB-type DNA segregation proteins are CTP-dependent molecular switches. Cell 179: 1512-1524. *co-corresponding authors
Benevides Martins G, Giacomelli G, Goldbeck O, Seibold GM, Bramkamp M (2019) Substrate-dependent cluster density dynamics of Corynebacterium glutamicum phosphotransferase system permeases. Mol Microbiol 111: 1335-1354.
Brameyer S, Rösch TC, El Andari J, Hoyer E, Schwarz J, Graumann PL, Jung K (2019) DNA-binding directs the localization of a membrane-integrated receptor of the ToxR family. Commun Biol 2: 4.
Ferreira JL, Gao FZ, Rossmann FM, Nans A, Brenzinger S, Hosseini R, Wilson A, Briegel A, Thormann KM, Rosenthal PB, Beeby M (2019) γ-proteobacteria eject their polar flagella under nutrient depletion, retaining flagellar motor relic structures. PLoS Biol 17: e3000165.
Hernandez-Tamayo R, Oviedo-Bocanegra LM, Fritz G, Graumann PL (2019) Symmetric activity of DNA polymerases at and recruitment of exonuclease ExoR and of PolA to the Bacillus subtilis replication forks. Nucleic Acids Res 47: 8521-8536.
Hinzpeter F, Tostevin F, Gerland U (2019) Regulation of reaction fluxes via enzyme sequestration and co-clustering. J Royal Soc Interface 16: 20190444.
Jung A, Raßbach A, Pulpetta RL, van Teeseling MCF, Heinrich K, Sobetzko P, Serrania J, Becker A, Thanbichler M (2019) Two-step chromosome segregation in the stalked budding bacterium Hyphomonas neptunium. Nat Commun 10: 3290.
Kober M, Bergeler S, Frey E (2019) Can a flux-based mechanism explain positioning of protein clusters in three-dimensional cell geometry? Biophys J 117: 420-428.
Pfeiffer D, Toro-Nahuelpan M, Bramkamp M, Plitzko JM, Schüler D (2019) The polar organizing protein PopZ is fundamental for proper cell division and segregation of cellular content in Magnetospirillum gryphiswaldense. mBio 10: e02716-18.
Ramm B, Heermann T, Schwille P (2019) The E. coli MinCDE system in the regulation of protein patterns and gradients. Cell Mol Life Sci 76: 4245-4273.
Ramm B, Schwille P (2019) In vitro reconstitution of the bacterial cytoskeleton: expected and unexpected new insights. Microb Biotechnol 12: 74-76.
Romero H, Rösch TC, Hernández-Tamayo R, Lucena D, Ayora S, Alonso JC, Graumann PL (2019) Single molecule tracking reveals functions for RarA at replication forks but also independently from replication during DNA repair in Bacillus subtilis. Sci Rep 9: 1997.
Szadkowski D, Harms A, Carreira LAM, Wigbers M, Potapova A, Wuichet K, Keilberg D, Gerland U, Søgaard-Andersen L (2019) Spatial control of the GTPase MglA by localized RomR-RomX GEF and MglB GAP activities enables Myxococcus xanthus motility. Nat Microbiol 4: 1344-1355.
Toro-Nahuelpan M, Corrales-Guerrero L, Zwiener T, Osorio-Valeriano M, Müller FD, Plitzko JM, Bramkamp M, Thanbichler M*, Schüler D* (2019) A gradient-forming MipZ protein mediating the control of cell division in the magnetotactic bacterium Magnetospirillum gryphiswaldense. Mol Microbiol 112: 1423-1439. *co-corresponding authors
Toro-Nahuelpan M, Giacomelli G, Raschdorf O, Bork S, Plitzko JM, Bramkamp M, Schüler D, Müller FD (2019) MamY is a membrane-bound protein that aligns magnetosomes and the motility axis of helical magnetotactic bacteria. Nat Microbiol 4: 1978-1989.
Wucher BR, Bartlett TM, Hoyos M, Papenfort K, Persat A, Nadell CD (2019) Vibrio cholerae filamentation promotes chitin surface attachment at the expense of competition in biofilms. Proc Natl Acad Sci USA 116: 14216-14221.
2018
Altuvia S, Storz G, Papenfort K (2018). Cross-regulation between bacteria and phages at a posttranscriptional level. Microbiol Spectr 6: RWR-0027-2018.
Bergeler S, Frey E (2018) Regulation of Pom cluster dynamics in Myxococcus xanthus. PLoS Comput Biol 14: e1006358.
Brenzinger S, Pecina A, Mrusek D, Mann P, Völse K, Wimmi S, Ruppert U, Becker A, Ringgaard S, Bange G, Thormann KM (2018) ZomB is essential for flagellar motor reversals in Shewanella putrefaciencs and Vibrio parahaemolyticus. Mol Microbiol 109: 694-709.
Checcucci A, diCenzo GC, Ghini V, Bazzicalupo M, Becker A, Decorosi F, Döhlemann J, Fagorzi C, Finan TM, Fondi M, Luchinat C, Turano P, Vignolini T, Viti C, Mengoni A (2018) Creation and characterization of a genomically hybrid strain in the nitrogen-fixing symbiotic bacterium Sinorhizobium meliloti. ACS Synth Biol 7: 2365-2378.
Glock P, Ramm B, Heermann T, Kretschmer S, Schweizer J, Mücksch J, Alagöz G, Schwille P (2018) Stationary patterns in a two-protein reaction-diffusion system. ACS Synth Biol 8: 148-157.
Gnesotto F, Mura F, Gladrow J, Broedersz C (2018) Broken detailed balance and non-equilibrium dynamics in living systems: a review. Rep Prog Phys 81: 066601.
Halatek J, Brauns F, Frey E (2018) Self-organization principles of intracellular pattern formation. Philos Trans R Soc Lond B Biol Sci 373: 20170107.
Herzog R, Paperfort K (2018) Transcriptomic approaches for studying quorum sensing in Vibrio cholerae. Methods Enzymol 612: 303-342.
Karniel A, Mrusek D, Steinchen W, Dym O, Bange G, Bibi E (2018) Co-translational folding intermediate dictates membrane targeting of the signal recognition particle receptor. J Mol Biol 430: 1607-1620.
Litschel T, Ramm B, Maas R, Heymann M, Schwille P (2018) Beating vesicles: encapsulated protein oscillations cause dynamic membrane deformations. Angew Chem Int Ed Engl 57: 16286-16290.
Lucena D, Mauri M, Schmidt F, Eckhardt B, Graumann PL (2018) Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns. BMC Biol 16: 97.
Miermans CA, Broedersz CP (2018) Bacterial chromosome organization by collective dynamics of SMC condensins. J R Soc Interface 15: 20180495.
Miyagi A, Ramm B, Schwille P, Scheuring S (2018) High-speed AFM reveals the inner workings of the MinDE protein oscillator. Nano Lett 18: 288-296.
Motz M, Jung K (2018) The role of polyproline motifs in the histidine kinase EnvZ. PLoS One 13: e0199782.
Najafi J, Shaebani MR, John T, Altegoer F, Bange G, Wagner C (2018). Flagellar number governs bacterial spreading and transport efficiency. Sci Adv 4: eaar6425.
Pollard AM, Sourjik V (2018) Transmembrane region of bacterial chemoreceptor is capable of promoting protein clustering. J Biol Chem 293: 2149-2158.
Qi F, Motz M, Jung K, Lassak J, Frishman D (2018) Evolutionary analysis of polyproline motifs in Escherichia coli reveals their regulatory role in translation. PLoS Comp Biol 14: e1005987.
Ramm B, Glock P, Mücksch J, Blumhardt P, Garcia-Soriano DA, Heymann M, Schwille P (2018) The MinDE system is a generic spatial cue for membrane protein distribution in vitro. Nat Commun 9: 3942.
Ramm B, Glock P, Schwille P (2018) In vitro reconstitution of self-organizing protein patterns on supported lipid bilayers. J Vis Exp 137: e58139.
Richter A, Hölscher T, Pausch P, Sehrt T, Brockhaus F, Bange G, Kovacs AT (2018) Hampered motility promotes the evolution of wrinkly phenotype in Bacillus subtilis. BMC Evol Biol 18: 155.
Robledo M, Schlüter JP, Loehr LO, Linne U, Albaum S, Jiménez-Zurdo JI, Becker A (2018) An sRNA and cold shock protein homolog-based feedforward loop post-transcriptionally controls cell cycle master regulator CtrA. Front Microbiol 9: 763.
Schumacher D, Søgaard-Andersen L (2018) Fluorescence live-cell imaging of the complete vegetative cell cycle of the slow-growing social bacterium Myxococcus xanthus. J Vis Exp 136: e57860.
Stockmar I, Feddersen H, Cramer K, Gruber S, Jung K, Bramkamp M, Shin JY (2018) Optimization of sample preparation and green color imaging using the mNeonGreen fluorescent protein in bacterial cells for photoactivated localization microscopy. Sci Rep 8: 10137.
Walter JC, Walliser NNO, David G, Geniet F, Palmeri J, Parmeggiani A, Wingreen NS and Broedersz C (2018) Looping and clustering model for the organization of protein-DNA complexes on the bacterial genome. New J Phys 20: 035002.
2017
Schubert K, Sieger B, Meyer F, Giacomelli G, Böhm K, Rieblinger A, Lindenthal L, Sachs N, Wanner G, Bramkamp M (2017) The antituberculosis drug ethambutol selectively blocks apical growth in CMN group bacteria. mBio 8: e02213–16.
Böhm K, Meyer F, Rhomberg A, Kalinowski J, Donovan C, Bramkamp M (2017) Novel chromosome organization pattern in Actinomycetales–overlapping replication cycles combined with diploidy. mBio 8: e00511–17.
Bach JN, Giacomelli G, Bramkamp M (2017) Sample preparation and choice of fluorophores for single and dual color photo–activated localization microscopy (PALM) with bacterial cells. Methods Mol Biol 1563: 129–141.
Döhlemann J, Wagner M, Happel C, Carrillo M, Sobetzko P, Erb TJ, Thanbichler M, Becker A (2017) A family of single copy repABC-type shuttle vectors stably maintained in the alpha-proteobacterium Sinorhizobium meliloti. ACS Synth Biol 6: 968-984.
Lin L, Osorio-Valeriano M, Harms A, Søgaard-Andersen L, Thanbichler M (2017) Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus. Nat Commun 8: 1817.
Schumacher D, Bergeler S, Harms A, Vonck J, Huneke-Vogt S, Frey E, Søgaard-Andersen L (2017) The PomXYZ proteins self-organize on the bacterial nucleoid to stimulate cell division. Dev Cell 41: 299-314.
Schumacher D, Søgaard-Andersen L (2017) Regulation of cell polarity in motility and cell division in Myxococcus xanthus. Annu Rev Microbiol 71: 61-78.